Hematoxylin-Eosin (H&E) staining has been used for more than a century and is still essential for identifying various tissue types and the morphological changes that form the basis of modern cancer diagnosis. For routine diagnosis, pathologists prefer H&E when observing the details of cell and tissue structure. H&E can display a wide range of cytoplasm, nucleus and extracellular matrix characteristics, for this reason, almost all teaching texts use H&E images.