Protein is a complex organic compound composed of amino acids. Due to the hydrophilicity, hydrophobicity, positive charge, negative charge and other characteristics of amino acid residues, it can be folded into a precise 3D structure through the interaction between the residues. The sequence of amino acids determines the unique 3D structure of each protein and its specific functions. Protein participates in almost every process in the cell and is necessary to maintain the structure and function of human tissues and organs. Protein folding errors can induce a variety of degenerative diseases, such as Parkinson’s disease, Alzheimer’s disease, frostbite, and senile heart failure. Therefore, imaging analysis of the folding process of proteins is of great significance for the early diagnosis and treatment of diseases.